In a classical multiplication stage, the culture supplies shoots for subsequent propagation, as well as material required to maintain the stock.

The sterile explants obtained in stage 1, once grown, will be transferred regularly to fresh medium. The main goal in the multiplication stage is the development of shoot formation by the sprouting of axillary buds. These shoots will be divided and transferred subsequently onto fresh medium until the desired number of plants is produced. This transfer of the shoots is labour intensive and takes place under sterile laboratory conditions. During this production process great care is taken to maintain the axenic (contaminant-free) status of the culture, utilising a range of strict quality assurance methods.